Resources

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An introduction to optical super-resolution microscopy for the adventurous biologist
J. Vangindertael, R. Camacho, W. Sempels, H. Mizuno , P. Dedecker, and K. P. F. Janssen

Methods and Applications in Fluorescence 6:022003 (2018)

https://doi.org/10.1088/2050-6120/aaae0c

This is a great overview of super resolution methods targeted at biologists. The article includes an introduction with a short history of the microscope and a basic primer on optics before moving on to more advanced topics.

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A quick guide to light microscopy in cell biology
K. Thorn

Molecular Biology of the Cell 27(2):219-222

https://doi.org/10.1091/mbc.E15-02-0088

Short overview of light microscopy for cell biology. A nice guide to the major concepts.

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The cell biologist’s guide to super-resolution microscopy

G. Jacquemet, A. Carisey, H. Hamidi, R. Henriques, and C. Leterrier

Journal of Cell Science 133:jcs240713

https://doi.org/10.1242/jcs.240713

Easy to read summary of major super-resolution methods highlighting key advantages and disadvantages.

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Super-resolution microscopy demystified

L. Schermelleh, A. Ferrand, T. Huser, C. Eggeling, M. Sauer, O. Biehlmaier, and G. Drummen

Nature Cell Biology 21:72-84 (2019)

https://doi.org/10.1038/s41556-018-0251-8

Guide to selecting a super-resolution method based on the biological question.

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Chemistry of Photosensitive Fluorophores for Single-Molecule Localization Microscopy

F. Jradi and L. Lavis

ACS Chemical Biology 14:1077-1090 (2019)

https://doi.org/10.1021/acschembio.9b00197

Nice chemistry primer on probe choice in localization based microscopy. Includes a nice table summarizing fluorescent proteins and their properties.

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Precisely and accurately localizing single emitters in fluorescence microscopy

H. Deschout, F. Zanacchi, M. Mlodzianoski, A. Diaspro, J. Bewersdorf, S. Hess, and K. Braeckmans

Nature Methods 11(3):253-266 (2014)

https://doi.org/10.1038/nmeth.2843

A more technical review of localization precision for imaging and single particle tracking.

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Tutorial: guidance for quantitative confocal microscopy

J. Jonkman, C. Brown, G. Wright, K. Anderson, and A. North

Nature Protocols 15:1585–1611 (2020)

https://doi.org/10.1038/s41596-020-0313-9

Overview of confocal microscopy and important considerations for quantitative imaging.

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About samples, giving examples: Optimized Single Molecule Localization Microscopy

Angélique Jimenez, Karoline Friedl, Christophe Leterrier

Methods 174:100–114 (2019)

https://doi.org/10.1016/j.ymeth.2019.05.008

Detailed information about cell culture, cover glass cleaning, antibody selection and imaging workflow for super-resolution microscopy.

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Appreciating the small things in life: STED microscopy in living cells

Alexander Stockhammer and Francesca Bottanelli

Journal of Physics D: Applied Physics 54:033001 (2021)

https://doi.org/10.1088/1361-6463/abac81

A nice guide to live cell STED imaging including a table summarizing live cell compatible STED dyes and relevant combinations for multi-color imaging.